Device

Part:BBa_K1491034:Design

Designed by: Danielle Peters   Group: iGEM14_Carnegie_Mellon   (2014-09-26)


Constitutive T7 RNAP with YFPH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Constitutive T7 RNAP with YFP for quantification of T7 RNAP


Source

The promoter is BBa_J23115, the RBS is BBa_B0034, the T7 RNA polymerase is BBa_K145001, the YFP is BBa_K864100

References